| Congresso Brasileiro de Microbiologia 2023 | Resumo: 392-1 | ||||
Resumo:ABSTRACT: Salmonella sp. is potentially identified as a cause of disease in humans and animals, its spread through the poultry production chain can occur due to failures during the handling, transportation and technological process of slaughter, contributing to cross-contamination and a possible presence of in the final product.The objective of this study was to verify the distribution of the main serovars of Salmonella sp. present in broiler slaughter. The study was carried out in a large slaughterhouse qualified for export located in the South of Braziland during 20 weeks samples of chicken carcasses (15 carcasses per week) were collected by surface rinsing of the carcasses in seven stages of the slaughter process: pre-scalding, post-scalding, post-penning, post-evisceration before PCC 1B, post-evisceration after PCC 1B, post-final shower and post-chiller totaling 2,100 samples.A total of 10 isolates were selected per week (selecting at least 1 isolate per point, if any, always performing a proportion according to the total number of isolates from each point), totaling 211 isolates of Salmonella spp. The isolates were serotyped by the Adolfo Lutz Institute - IAL/SP. Five Salmonella serovars were identified, with S. Heidelberg being the most prevalent with 91.5% (n=193), followed by S. Infantis 5.7% (n=12), S. Minnesota 1.4% (n=3), S. Coeln 0.9% (n=2) and S. Saintpail 0.5% (n=1). During the 20 weeks, S. Heidelberg was identified at various points, with pre- calping being the most prevalent. S. Infantis was detected only at weeks 15, 16 and 17, with a highlight at week 15, where it was identified at pre-scalding, post-scalding, post-evisceration before PCC 1B, post-evisceration after PCC 1B, post final shower and post-chiller. Two isolates of S. Minnesota were obtained from week three (post-evisceration points before PCC 1B, post-evisceration after PCC 1B) and one at week 17 (pre-scalding). The other two serovars: Coeln and Saintpail were identified at week 20 (pre-scald and post final shower) and 16 (pre-scald) respectively. According to European data, the serovars of S. Enteritidis, Typhimurium, monophasic Typhimurium (1,4,[5],12:i:-), Infantis and Derby, were the most involved in human infections, in the current study only S. Infantis was identified, however the other serovars identified are important for public health, since all are potentially causative of disease and related to a greater resistance to antimicrobials. We concluded that the slaughter of chicken allowed a huge diversity of serotypes of Salmonella during the process. Most of the isolates being of the Heidelberg serovar may be related to the control and vaccination of commercial poultry against Typhimurium and Enteritidis, reducing these serovars and consequently selecting the persistence of others in the slaughter process.
Keywords: Cross-contamination, Persistence; Salmonella persistence
Development Agency: The authors thank the National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq, Brasília, DF, Brazil), the Coordination for the Improvement of Higher Education Personnel ( Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES, Brasília, DF, Brazil – Code 001). Palavras-chave: Cross-contamination, Persistence, Salmonella persistence Agência de fomento: CNPq e CAPES | |||||